Device

Part:BBa_K4408009:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)


Pthl-adhE2-thl-hbd, Expression of adhE2, thl and hbd with Pthl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3907
    Illegal SapI.rc site found at 3731


Design Notes

In our program, we use this device to construct and optimize the synthesis pathways of butanol and butyrate in Clostridium tyrobutyricum. By introducing adhE2，butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Enzyme encoded by thl catalyzes acetyl-CoA into acetoacetyl-CoA which is further transformed to 3-hydroxybutytyl-CoA by hbd encoded protein. These two enzymes are key enzymes in the synthesis pathway of butyl acid in Clostridium tyrobutyricum. By overexpressing thl and hbd, we enhanced the production of butyrate in Clostridium tyrobutyricum. Pthl starts the transcription and Cpa fdx terminator ends the transcription.

Source

Pthl is based on part BBa_K3443002. RBS1 is based on BBa_K103015. adhE2 is based on part BBa_K1462060. thl is based on part BBa_K3443003. hbd is based on part BBa_M1350. Cpa fdx terminator is based on part BBa_K3585002.

References